SREL Reprint #3124

Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus

Stacey L. Lance1,2, Cris Hagen2, Travis C. Glenn1, Nicole A. Freidenfelds3, and Tracy Langkilde3

1Department of Environmental Health Science, University of Georgia, Athens, GA 30602, USA
2Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
3Department of Biology, The Pennsylvania State University, 208 Mueller Lab, University Park, PA 16802, USA

Abstract: We isolated and characterized 17 microsatellite loci from the eastern fence lizard, Sceloporus undulatus. Loci were screened in 24 individuals of S. undulatus. The number of alleles per locus ranged from 8 to 22, observed heterozygosity ranged from 0.174 to 0.913, and the probability of identity values ranged from 0.008 to 0.1. These new loci provide tools for examining the population genetics of eastern fence lizards and for assessing the evolutionary impacts the invasive red imported fire ant has on the lizard.

Key words: Eastern fence lizard, Sceloporus, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3124

Lance, S. L., C. Hagen, T. C. Glenn, N. A. Freidenfelds, and T. Langkilde. 2009. Development and characterization of seventeen polymorphic microsatellite loci in the eastern fence lizard, Sceloporus undulatus. Conservation Genetics Resources 1: 233-236.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).