SREL Reprint #3187

Twelve novel microsatellite markers for the marbled salamander, Ambystoma opacum

Schyler O. Nunziata1,2, David E. Scott1, Kenneth L. Jones3, Cris Hagen1, and Stacey L. Lance1

1Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
2Department of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475, USA
3Department of Biochemistry and Molecular Genetics, School of Medicine,
University of Colorado, Aurora, CO 80045, USA

Abstract: We isolated and characterized a total of 12 microsatellite loci from the marbled salamander, Ambystoma opacum. Loci were screened in 24 individuals from a single location on the Savannah River Site in South Carolina. The number of alleles per locus ranged from 3 to 15, observed heterozygosity ranged from 0.167 to 0.917, and the probability of identity values ranged from 8.5 x 10-2 to 3.2 x 10-1. These new loci will provide tools for examining the genetic diversity, structure, and mating system of A. opacum populations.

Keywords: Ambystoma, Marbled salamander, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3187

Nunziata, S. O., D. E. Scott, K. L. Jones, C. Hagen, and S. L. Lance. 2011. Twelve novel microsatellite markers for the marbled salamander, Ambystoma opacum. Conservation Genetic Resources 2011(3): 773-775.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).