SREL Reprint #3225

A new set of microsatellite loci for Laptonycteris yerbabuenae and cross species amplification with other glossophagines

Jose Antonio Romero-Meza1, Stacey L. Lance2, and Jorge Ortega1

1Laboratorio de Ictiología y Limnología, Posgrado en Ciencias Quimicobiológicas, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Sto. Tomas, 11340 Mexico, DF, Mexico
2Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802 USA

Abstract: A technique based on 454 sequencing of an enriched library was used to construct genomic libraries highly enriched for microsatellite loci for Leptonycteris yerbabuenae. Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. We probed ascertainment bias in 5 different glossophagines (L. nivalis, Glossophaga leachii, G. soricina, Anoura geoffroyi, and Choeronycteris mexicana), indicating their potential utility for suitable studies of population genetics and other related analyses. Levels of expected heterozygosity were medium–low for all markers (mean HE = 0.147, range 0.008–0.46).

Keywords: 454 Sequencing, Ascertainment bias, Glossophagines, Leptonycteris yerbabuenae, Microsatellites

SREL Reprint #3225

Romero-Meza, J. A., S. L. Lance, and J. Ortega. 2012. A new set of microsatellite loci for Laptonycteris yerbabuenae and cross species amplification with other glossophagines. Conservation Genetic Resources 2012(4): 291-294.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).