SREL Reprint #3156

Isolation and characterization of 17 polymorphic microsatellite loci in the kangaroo mouse, genus Microdipodops (Rodentia: Heteromyidae)

Stacey L. Lance1, Jessica E. Light2, Kenneth L. Jones3, Cris Hagen1, and John C. Hafner4

1Savannah River Ecology Laboratory, Aiken, SC 29802, USA
2Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX 77843, USA
3Georgia Genomics Facility, University of Goergia, Athens, GA 30602, USA
4Moore Laboratory of Zoology and Department of Biology, Occidental College, Los Angeles, CA 90041, USA

Abstract: We isolated and characterized 17 microsatellite loci from kangaroo mice, Microdipodops megacephalus and M. pallidus. Loci were screened in 24 individuals from 21 general localities across their distributional range in the Great Basin Desert. In total, the number of alleles per locus ranged from 4 to 16, observed heterozygosity ranged from 0.333 to 1, and the probability of identity values ranged from 0.013 to 1. These loci provide new tools for examining the biogeographic history and population dynamics of Microdipodops in the context of molecular ecology.

Keywords: Microdipodops, Kangaroo mouse, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3156

Lance, S. L., J. E. Light, K. L. Jones, C. Hagen, and J. C. Hafner. 2010. Isolation and characterization of 17 polymorphic microsatellite loci in the kangaroo mouse, genus Microdipodops (Rodentia: Heteromyidae). Conservation Genetics Resources 2010(2): 139-141.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).