SREL Reprint #3082

Isolation and characterization of microsatellite loci in the Guanacaste tree, Enterolobium cyclocarpum

Maureen B. Peters1, Cris Hagen1, Dorset W. Trapnell2, J. L. Hamrick3,
Oscar Rocha4, Peter E. Smouse5, and Travis C. Glenn1

1Savannah River Ecology Laboratory, University of Georgia, PO Drawer E, Aiken, SC 29802, USA
2Institute of Ecology, University of Georgia, Athens, GA 30602, USA
3Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
4Department of Biological Sciences, Kent State University, Kent, OH 44242, US
5Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, NJ 08901, USA

Abstract: We isolated nine microsatellite loci from the Guanacaste tree (Enterolobium cyclocarpum) and optimized them for future research on breeding populations of this species. Loci were screened across 53 individuals from one population and were shown to be variable with the number of alleles per locus ranging from five to 15. Polymorphic information content ranged from 0.420 to 0.900 and observed heterozygosity ranged from 0.547 to 0.906.

Keywords: Enterolobium cyclocarpum, Guanacaste tree, microsatellites, parentage analysis, PCR, pollen dispersal, population structure

SREL Reprint #3082

Peters, M. B., C. Hagen, D. W. Trapnell, J. L. Hamrick, O. Rocha, P. E. Smouse and T. C. Glenn. 2008. Isolation and characterization of microsatellite loci in the Guanacaste tree, Enterolobium cyclocarpum. Molecular Ecology Resources 2008(8): 129-131.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).