SREL Reprint #2712

Polymorphic tetranucleotide microsatellite DNA loci from the southern dusky salamander (Desmognathus auriculatus)

Dean A. Croshaw1,2 and Travis C. Glenn1,3

1Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
2Department of Biological Sciences, University of New Orleans, New Orleans, LA 70148, USA
3Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA

Abstract: We describe polymerase chain reaction (PCR) primers and amplification conditions for seven tetranucleotide microsatellite DNA loci isolated from the southern dusky salamander (Desmognathus auriculatus). Primers were tested on 16 individuals from one population in Aiken County, South Carolina. We detected an average of 6.57 alleles per locus, an observed heterozygosity range of 0.44-0.94, and high polymorphic information contents (mean of 0.68).

Keywords: Desmognathus auriculatus, dusky salamanders, microsatellites, polymerase chain reaction primers, short tandem repeat, simple sequence repeat

SREL Reprint #2712

Croshaw, D. A. and T. C. Glenn. 2003. Polymorphic tetranucleotide microsatellite DNA loci from the southern dusky salamander (Desmognathus auriculatus). Molecular Ecology Notes 3: 623-625.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).