SREL Reprint #3237

Development of polymorphic microsatellite markers for blue king crab (Paralithodes platypus)

Jennifer L. Stoutamore1, Cara N. Love2, Stacey L. Lance2, Kenneth L. Jones3, and David Tallmon1,4

1Fisheries Division, University of Alaska Fairbanks, Juneau, AK 99801, USA
2Savannah River Ecology Laboratory, University of Georgia, Aiken, SC 29802, USA
3Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine,
Aurora, CO 80045, USA
4Biology and Marine Biology Program, University of Alaska Southeast, Juneau, AK 99801, USA

Abstract: We isolated and characterized a total of 23 microsatellite loci from the blue king crab, Paralithodes platypus. Loci were screened in 24 individuals from St. Matthew Island. The number of alleles per locus ranged from 4 to 17, observed heterozygosity ranged from 0.050 to 1.000, and the probability of identity values ranged from 0.015 to 0.339. These new loci will provide tools for examining the genetic population structure of the species throughout its range.

Keywords: Paralithodes, Illumina, Microsatellite, PAL_FINDER, PCR primers, SSR

SREL Reprint #3237

Stoutamore, J. L., C. N. Love, S. L. Lance, K. L. Jones, and D. Tallmon. 2012. Development of polymorphic microsatellite markers for blue king crab (Paralithodes platypus). Conservation Genetic Resources 2012(4): 897-899.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).