SREL Reprint #3074

Development and characterization of microsatellite loci in the eastern chipmunk (Tamias striatus)

Maureen B. Peters1, Julie L. Glenn1, Pam Svete1, Cris Hagen1, Olga V. Tsyusko1,
Patricia Decoursey2, Mélissa Lieutenant-Gosselin3, Dany Garant3,
and Travis C. Glenn1,2

1Savannah River Ecology Laboratory, Institute of Ecology, The University of Georgia, PO Drawer E, Aiken, SC 29802, USA
2Department of Biological Sciences, University of South Carolina, Columbia, SC 29802, USA
3Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada J1K 2R1

Abstract: We isolated and characterized 16 microsatellite loci in the eastern chipmunk, Tamias striatus. The loci were screened across 25 individuals from one population and shown to be polymorphic with the number of alleles per locus ranging from two to 17. Polymorphic information content ranged from 0.212 to 0.887 and observed heterozygosity ranged from 0.200 to 0.960. Only two loci (Chip 10 and Chip 25) deviated from Hardy–Weinberg equilibrium.

Keywords: Eastern chipmunk, microsatellites, parentage analysis, PCR, population structure, Tamias striatus

SREL Reprint #3074

Peters, M. B., J. L. Glenn, P. Svete, C. Hagen, O. V. Tsyusko, P. Decoursey, M. Lieutenant-Gosselin, D. Garant and T. C. Glenn. 2007. Development and characterization of microsatellite loci in the eastern chipmunk (Tamias striatus). Molecular Ecology Notes 2007(7): 877-879.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).