SREL Reprint #3075

Fifteen polymorphic microsatellite DNA loci from Hawaii’s Metrosideros polymorpha (Myrtaceae: Myrtales), a model species for ecology and evolution

Nicholas G. Crawford1, Cris Hagen1, Heather F. Sahli2, Elizabeth A. Stacy2,
and Travis C. Glenn1

1Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA
2Department of Biology, University of Hawaii at Hilo, 200 W. Kawili Street, Hilo, HI 96720, USA

Abstract: We developed 15 polymorphic microsatellite loci from the Hawaiian tree Metrosideros polymorpha. These loci were screened against two varieties from several populations and from 23 individuals from one mid-elevation population on Hawaii Island. Loci were variable with the number of alleles per locus ranging from three to 24. Polymorphic information content ranged from 0.222 to 0.941, and observed heterozygosity ranged from 0.261 to 0.955.

Keywords: Hawaii, Metrosideros, microsatellite, PCR primers, polymorpha, SSR, STR, tree

SREL Reprint #3075

Crawford, N. G., C. Hagen, H. F. Sahli, E. A. Stacy and T. C. Glenn. 2008. Fifteen polymorphic microsatellite DNA loci from Hawaii's Metrosideros polymorpha (Myrtaceae: Myrtales), a model species for ecology and evolution. Molecular Ecology Resources 2008(8): 308-310.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).