SREL Reprint #2690

Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin)

J. Susanne Hauswaldt1,2 and Travis C. Glenn1,2

1Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA
2Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC 29802, USA

Abstract: We describe polymerase chain recation (PCR) primers and conditions to amplify one dinucleotide and five tetranucleotide microsatellite DNA loci isolated from the Diamondback terrapin (Malaclemys terrapin). The PCR primers were tested on 21 terrapins from Cape Romain, SC, USA. The microsatellite primers developed yielded a high number of alleles (8-14) and high observed heterozygosities (0.57-1.0).

Keywords: dinucleotide repeats, Malaclemys terrapin, microsatellites, primer, SSR, tetranucleotide repeats

SREL Reprint #2690

Hauswaldt, J. S. and T. C. Glenn. 2003. Microsatellite DNA loci from the diamondback terrapin (Malaclemys terrapin). Molecular Ecology Notes 3:174-176.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).