SREL Reprint #3157

Development and characterization of twelve polymorphic microsatellite loci in the Bog Copper, Lycaena epixanthe

Sarah P. Flanagan1, W. Herbert Wilson1, Kenneth L. Jones2, and Stacey L. Lance3

1Department of Biology, Colby College, Waterville, ME 04901, USA
2Georgia Genomics Facility, University of Georgia, Athens, GA 30602, USA
3Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC 29802, USA

Abstract: We isolated and characterized 12 microsatellite loci from the Bog Copper, Lycaena epixanthe. Loci were screened in 24 individuals from one Maine location. Alleles per locus ranged from 3 to 12, observed heterozygosity ranged from 0.042 to 0.857, and the probability of identity values ranged from 0.032 to 0.780. These loci provide tools for examining the population genetics of Bog Coppers across a fragmented landscape.

Keywords: Bog Copper, Butterfly, Lycaena, Microsatellite, PCR primers, SSR, STR

SREL Reprint #3157

Flanagan, S. P., W. H. Wilson, K. L. Jones, and S. L. Lance. 2010. Development and characterization of twelve polymorphic microsatellite loci in the Bog Copper, Lycaena epixanthe. Conservation Genetics Resources 2010(2): 159-161.

This information was provided by the University of Georgia's Savannah River Ecology Laboratory (srel.uga.edu).