Pipette Training

Pipette Training

Pipette Training (Video)

A. Pipetting Test

Pipette sizes used in this test:

2 µl, 10 µl, 40 µl, 200 µl, and 1000 µl

1. Place a small weighing boat on the weighing scale, and zero the scale. Use a 1000 µl pipette to draw 650 µl of water into the weighing boat

If accurate, 0.65 g will be shown on the scale.

2. Draw 30 µl of water using the 40 µl pipette -using a weighing boat

If accurate, 0.03 g will be shown on the scale.

3. Draw 170 µl of water using a 200 µl pipette - using a weighing boat

If accurate, 0.17 g will be shown on the scale.

4. Draw 10 µl of water using a 10 µl pipette and place it in a small microcentrifuge tube. Repeat 5 times.

If accurate, 0.06 g will be shown on the scale.

5. Draw 2 µl of water using a 2 µl pipette and place it in a small microcentrifuge tube. Repeat 9 times.

If accurate, 0.02 g will be shown on the scale.