Functions of Common Electrophysiology Solutions

Functions of Common Electrophysiology Solutions

• • I+I: isoproterenol and IBMX

    • Isoproterenol

      • B2AR agonist

      • Increases cAMP

    • IBMX

      • Phosphodiesterase (PDE) inhibitor

        • PDE normally degrades cAMP and cGMP

      • Increases cAMP and cGMP

    • Role of cAMP

      • Activates protein kinase A (PKA)

      • PKA can phosphorylate CFTR

      • Phosphorylation of CFTR activates/opens it

      • Activates other ion channels as well

    • Overall: used to activate ion channels

  • 2ME: 2-mercaptoethanol

    • Reduces disulfide bonds

    • Helps stabilize and maintain activity of protein

    • Reduces variability of results by making the CFTR proteins homogenous 

  • DTT: 

    • Reduces disulfide bonds (more effectively than 2ME)

    • This is so we have a homogeneous population of the channels in the oocyte, so we can generalize results to a single channel and reduce variability in the results

  • GlyH101

    • CFTR inhibitor (dose dependent)

    • Open channel blocker

  • SCN-

    • Blocks CFTR

  • PEA

    • Trace amine

    • Activates TAAR1

• MTS reagents

  • https://www.caymanchem.com/product/16529 

  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737665/ 

  • Good experiment

    • Nothing happens in FR (stable, horizontal line)

    • I+I: channels activated, conductance increases (no more than ~100ish microsiemens), line goes up, plateau