Molecular Biology

The main goal of molecular biology in our lab is to mutate amino acids in our drug and gene therapy targets. The general approach is shown in this flow chart.

We often mutate the engogenous amino acid into a cysteine because cysteine can be covalently modified during an electrophysiology experiment with thiol-directed reagents and alkylating reagents. Obtaining pure (plasmid) DNA is also important for human cell transfection (CRISPR project) and viral transduction.

Equipment Protocols

Running the Big Centrifuge on Floor 2

Shaking Incubator Full Explanation

Using Basement Autoclave Video (45 seconds in)

Solutions

Gel extraction and DNA clean-up kit for PCR