Embedded Files
The goal of this subproject is to express Pertussis toxin protomer A together with TAAR1 to facilitate its coupling to GIRK channels. We purchased XE100 Pertussis toxin A protomer (Plasmid #16678) from Addgene to make mRNA. This plasmid has SP6 promoter and therefore the SP6 Transcription Kit can be used.
The gene that codes for Pertussis toxin A protomer is called TOX1_BORPE or ptxA.
It has the following sequence: MRCTRAIRQTARTGWLTWLAILAVTAPVTSPAWADDPPATVYRYDSRPPEDVFQNGFTAWGNNDNVLDHLTGRSCQVGSSNSAFVSTSSSRRYTEVYLEHRMQEAVEAERAGRGTGHFIGYIYEVRADNNFYGAASSYFEYVDTYGDNAGRILAGALATYQSEYLAHRRIPPENIRRVTRVYHNGITGETTTTEYSNARYVSQQTRANPNPYTSRRSVASIVGTLVRMAPVIGACMARQAESSEAMAAWSERAGEAMVLVYYESIAYSF
Pertussis toxin plasmid map for Snapgene:
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