SARS-CoV-2-S-RBD-sfGFP

6/08/21

The plasmid was retrieved from the mail room as a bacterial stab and stored in Fridge B.

6/09/21

Two plates of SARS-CoV-2-S-RBD-sfGFP_pcDNA3 were streaked and isolated for colonies by HS. The plates were placed in the incubator at 37C overnight.

6/10/21

Sequencing primers were designed and ordered for SARS-CoV-2-S-RBD-sfGFP_pcDNA3.

SARS-CoV-2-S-RBD-sfGFP_pcDNA3 was inoculated into 2 flasks by HS at 5pm.

6/11/21

SARS-CoV-2-S-RBD-sfGFP-1_pcDNA3 and SARS-CoV-2-S-RBD-sfGFP-2_pcDNA3 was midiprepped at 8am by HS. After the 100% ethanol step (step 8) no pellets formed so .95mL and 0.6mL of sodium acetate buffer was added to SARS-CoV-2-S-RBD-sfGFP_pcDNA3-1 and 2, respectively. The tubes were inverted 10 times and left overnight.

6/12/21

SARS-CoV-2-S-RBD-sfGFP_pcDNA3-1 and 2 were spun at 10,000g for 3 minutes by LC. The last ethanol step was completed and the pellets were left to dry over 2 nights.

6/14/21

Resuspended the pellets in 20uL of MBG Water by HS. Ran on a gel by HS.

6/15/21

Maxiprepped by LC. No large pellets formed after the 100% ethanol step, so 3.5mL and 3.4mL of sodium acetate buffer was added to SARS-CoV-S-RBD-sfGFP_pcDNA3-1 and 2, respectively. The solution is sitting overnight.

6/16/21

SARS-CoV-S-RBD-sfGFP_pcDNA3-1 and 2 from the midiprep on 6/11/21 were sent for sequencing. The sequencing is under 06/11/21. 

6/17/21

Analyzed on a gel by ST.

6/18/21

SARS-CoV-S-RBD-sfGFP_pcDNA3-1 and 2 from the maxiprep on 6/15/21 were sent for sequencing. The sequencing is under 6/16/21 sequencing. SARS-CoV-S-RBD-sfGFP_pcDNA3-1 has the best results from sequencing.