Oocyte Freezing

• 
  Freezing Protocols

  • Vitrification

  • Slow freezing

4/19/18

Vitrification solutions made with the following amounts:

• 10 mL total solution with 5M DMSO and 1M Mannitol

  • DMSO - 3.55 mL

  • Mannitol - 1.8035 g

  • MBSH Water - 6.45 mL

• With this 10 mL solution, 6 1.5 mL tubes were loaded with 1 mL each. 3 of these tubes placed in freezer C, box 5 (labeled “CV”) and 3 placed in -80 freezer, box Yohei (labeled “-80 V”)

Slow freezing solution made with the following amounts:

• 10 mL total solution with 1.5M DMSO and 0.2M Mannitol

  • DMSO - 1.09 mL

  • Mannitol - 0.4004 g

  • MBSH Water - 8.91 mL

• With this 10 mL solution, 6 1.5 mL tubes were loaded with 1 mL each. 3 of these tubes were placed in freezer C, box 5 (labeled “CS”) and 3 placed in -80 freezer, box Yohei (labeled “-80 S”)

The remaining 4 mL from both the vitrification and slow freeze solutions were left out on the red tube rack