Embedded Files
9/29/21
9/29/21
Purified with a Miniprep (Gel Picture and nanodrop concentration)
9/14/21
9/14/21
Maxiprep of L102C_CFTR_FLAG_pcDNA3.1
9/7/21
9/7/21
Redo transformation of L102C_CFTR_FLAG_pcDNA3.1
8/31/21
8/31/21
Transformation of L102C_CFTR_FLAG_pcDNA3.1
8/27/21
8/27/21
An error from Genewiz occurred in the plasmid they sent over. They resent a sample of the correct sequence.
8/06/21
8/06/21
Redone Circularization of wtCFR_FLAG_pcDNA3-1(1), wtCFTR_FLAG_pcDNA3-1 (2), wtCFTR_FLAG_pcDNA3-2 (1), and wtCFTR_FLAG_pcDNA3-2 (2)
8/05/21
8/05/21
Transcription of linear L102C_FLAG_pcDNA3
8/04/21
8/04/21
Circularization of wtCFR_FLAG_pcDNA3-1(1) and wtCFTR_FLAG_pcDNA3-2 (1)
8/02/21
8/02/21
Dpn1 Digestion of wtCFTR_FLAG_pcDNA3-1 and wtCFTR_FLAG_pcDNA3-2
8/01/21
8/01/21
Phenol Chloroform Extraction L102C_FLAG_pcDNA3-1 and L102C_FLAG_pcDNA3-2
7/30/21
7/30/21
Phenol Chloroform Extraction L102C_FLAG_pcDNA3-1 and L102C_FLAG_pcDNA3-2
7/29/21
7/29/21
Linearize L102C_FLAG_pcDNA3-1 and L102C_FLAG_pcDNA3-2
7/27/21
7/27/21
Sent L102C_FLAG_pcDNA3-1 and L102C_FLAG_pcDNA3-2 for sequencing
7/27/21
7/27/21
Ran PCR with L102C_FLAG_pcDNA3-1 and L102C_FLAG_pcDNA3-2.
7/20/21
7/20/21
Maxiprepped L102C_FLAG_pcDNA3-1 and L102C_FLAG_pcDNA3-2
7/12-15/21
7/12-15/21
Transformed L102C_FLAG_pcDNA3 three separate times
Linearize
7/08/21
7/08/21
Received L102C_FLAG_pcDNA3 from Genewiz and resuspended to be 100ng/uL and is in its original tube in Freezer A (red cap)
Page updated
Report abuse