Embedded Files
5/31/21
5/31/21
Thawed 1 tube of LM45 cells that were retrieved from the -80C freezer. 4 35mm plates were made with approximately 2mL of media per plate. The cells sat in the tissue culture hood for around 30 minutes while we turned the incubator on and adjusted it to the correct levels. The temperature was at 22C instead of 37C but has the proper O2 and CO2 levels, so the cells were placed in the incubator. They were at a low con-fluency and were not passaged. Plate 4 was pipetted into incorrectly and will be noted among further usage.
6/01/21
6/01/21
Cells were looked at under the microscope to see the confluency. They were not confluent enough so we are leaving them in the incubator until later.
6/02/21
6/02/21
Cells were looked at under the microscope to see the confluency.
Plate 1
Not ready for passaging.
Plate 2
Could be ready for passaging, but not necessary.
Plate 3
Not ready for passaging
Plate 4
Not ready for passaging. This culture only has circular cells which differs from the others.
6/03/21
6/03/21
Cells were looked at under the microscope to see the confluency.
Plate 1
Not ready for passaging.
Plate 2
Has the most growth, but isn't ready for passaging.
Plate 3
Over half the cells were attached to the bottom of the plate, but the rest were floating around. Not ready for passaging. Need to be monitored since cells are not adhering.
Plate 4
All cells are still circular. They might not be alive and growing.
6/04/21
6/04/21
Cells were looked at under the microscope to see the confluency. Plates 1 and 3 were passaged into 3 new plates each (1-1, 1-2, 1-3, 3-1, 3-2, 3-3)
Plate 1
100% confluent. Will be passaged today.
Plate 2 (Image 1)
Plate has come insistency in cell types and will not be passaged. Some cells are possibly dead.
Plate 2 (Image 2)
Possible scratch on the plate.
Plate 3
100% confluent. Will be passaged today
Plate 4
Cells are probably dead.
6/05/21
6/05/21
Plate 1-1 (Image 1)
Some cells were floating.
Plate 1-1 (Image 2)
There were about 3 rings in this cell culture.
Plate 1-2 (Image 1)
Some cells were floating.
Plate 1-2 (Image 2)
This anomaly only seemed to appear once in this cell culture.
Plate 1-3 (Image 1)
Some cells were floating.
Plate 1-3 (Image 2)
A large clump of cells.
Plate 1-3 (Image 3)
There seemed to be only 1 anomaly.
Plate 3-1
Some cells growth.
Plate 3-2
Some cells were floating.
Plate 3-3
Some cell growth.
6/07/21
All air bubbles from Saturday had dissipated by today. No cell growth was visible in any plate.
Plate 1-1
No visible cell growth.
Plate 1-2
No visible cell growth.
Plate 1-3
No visible cell growth.
Plate 3-1
No visible cell growth.
Plate 3-2
No visible cell growth.
Plate 3-3
No visible cell growth.
6/08/21
6/08/21
Cells were checked on and found that the growth media had evaporated out of the dish. These cells are dead and the reasoning behind that is attributed to the incubator. Bonnie is looking into reasons why.
Plate 1-1
Plate 1-2
Plate 1-3
Plate 3-1
Plate 3-2
Plate 3-3
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