Placed in the incubator at 6:10pm, will remove from the incubator between 9:10 and 10:10

• Began at 9:32am

• after adding TENS buffer and Sodium acetate it was cloudy. That's not supposed to happen but I'm not sure if it's a bad thing. We continued anyways.

62ng/uL - 4618.5ng/uL

10ng/uL - 3082.8 ng/uL

2ng/uL - 5496.4 ng/uL

• I preformed the protocol for miniprep on the maxiprep product.

• DNA cleanup was not successful, at the 100% ethanol precipitation stage, no pellet formed.

• I did not continue further than this, because this indicated that maxiprep had failed.

• Redoing transformation again.