Resuspension

November 19, 2021

1. Take tube with DNA pellet in 15 mL falcon tube and place the lid back on the tube (or with the tube)

2. Add 1 mL of MBG water (from Fridge A) STRAIGHT INTO FALCON TUBE

3. Cap back on

4. Vortex in the back of lab (get pellet entirely into solution)

5. Take cap off and pipette into ~1.5 mL tube (clear tube)

   1. Throw away falcon tube

   2. Label new tube with KCNJ5_FLAG_pcDNA 3.1 then on the side put MN AJH and 11/21

6. Place in Freezer A for storage