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KCNJ9 protein coding sequence was subcloned into pGHE using NEBuilder HiFi DNA Assembly Master Mix.
KCNJ9 protein coding sequence was subcloned into pGHE using NEBuilder HiFi DNA Assembly Master Mix.
1. Designing of PCR Primers - Created by Lucas Handlin
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2. PCR with pGHE TAAR1 and pENTR KCNJ9 as templates - Done by Lucas Handlin and Tina Ruedlin
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3. Confirmation of reaction by gel
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4. HiFi DNA Assembly Reaction
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5. Transformation and Miniprep
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6. Sanger Sequencing
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