A plasmid (pENTR) containing the protein coding sequence for human KCNJ9 was purchased from DNASU. KCNJ9 mRNA was produced as shown below. The mRNA is injected into oocytes to express human KCNJ9 channels in the plasma membrane.
1. Maxiprep of pENTR KCNJ9
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2. Subcloning of KCNJ9 into pGHE
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3. In vitro transcription
