Good vs. Bad Data

Following an electrophysiology experiment, the data are analyzed using pClamp Conversion software to produce the figures at the top.  After that, good experiments are analyzed using SigmaPlot to determine if they are publishable. CFTR channels are activated in I+I and channel conductance reaches a plateau when maximally activated.  Channel conductance should be steady before application of GlyH101, and decrease at high concentrations of GlyH-101.  The graph on the right has increasing conductance at low GlyH101 concentrations, so this is our first clue that the data are not useable.  Further analysis in SigmaPlot should result in a nice exponential curve, as seen on the left.  The data points on the right are not quite what we are looking for here.