Embedded Files
Bottleneck Experiments
Bottleneck Experiments
In order to see the experimental protocol and/or solutions used in this project, go to:
SCN Block
GlyH-101 Block
Anion Substitution
In this project, we are investigating how different anions including SCN and GlyH-101 interact with the narrowest part (bottleneck) of the CFTR channel pore.
As you can see above, there are 3 categories of experiments in this project:
SCN Block, GlyH-101 Block and Anion Substitution.
In each category we are investigating the impact of mutagenesis (L102C and R104C) and covlent modification of the engineered cysteine with MTS reagents and NEM.
ANION EXPERIMENT DATA
ANION EXPERIMENT DATA
Anion Experiment Data
Anion Experiment Data
This section is a quick link guide to all of the compiled data for the Anion experiments done from 2017-2020. The data is subdivided into sections based upon the flow chart found on the previous page. From these links, the calculated relative permeability as well as the raw data from the electrophysiology experiments. A table of the relative permeability of the different combinations can be found here.
R104C
MSTET- June 2017- March 2020
MSTES- April 2019 - February 2020
No Mod- April 2017 - February 2020
L102C
MTSET
DTT- May 2018
No DTT- November 2017 - August 2018
MTSEA- February 2020
No Mod- March 2017 - February 2020
NaSCN experiment data
NaSCN experiment data
NaSCN experiment data
NaSCN experiment data
This section is a quick link guide to all of the compiled data for the NaSCN experiments done from 2017-2020. The data is subdivided into sections based upon the flow chart found on the previous page. From these links, the raw data from the electrophysiology experiments along with sigma plots derived from the data. A table of the relative permeability of the different combinations can be found here.
WT- August 2018 - November 2018
R104C
MTSET- February 2020 - March 2020
MTSES- March 2019 - November 2019
No Mod- February 2019 - September 2019
L102C
MTSEA- October 2019 - November 2019
No Mod- April 2018 - July 2019
NEM- November 2018 - November 2019
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