Embedded Files
Transformed L102C_CFTR_FLAG_pcDNA3 and CFTR_pGHE (from 774ng/uL tube). Done by HS.
Results:
L102C_CFTR_FLAG_pcDNA3 from stock sent by GeneWiz.
This plate indicates that transformation was successful. There are a larger number of colonies here than hoped for, however, there are several colonies of good shape and size that could be used for a maxi-prep.
CFTR_pGHE from 774ng/uL tube currently in Freezer A.
This plate indicates that transformation took place. Unfortunately, there are a large number of colonies that makes finding a usable colony difficult. In combination with the 7/13/21 transformation, there should be enough colonies to have at least one successful maxi-prep.
Negative control of water was added to the culture plate and incubated.
This plate indicates that transformation was successful as no bacteria grew in this plate.
Page updated
Report abuse