Embedded Files
Transformation was done on 5/26/21 on LB +AMP Plates made on 5/26/21 by HS. Transformation done by HS.
KCNJ3-2 pGHE with a concentration between 741.8ng/uL and 2357.33ng/uL. DNA from Lane 2 on LMC052421B.
This plate indicates that circular DNA and that transformation occurred. The colonies are too small and too close together to be used for a maxi-prep. This is probably due to the concentration of the DNA being too high, or the amount of bacteria used in the transformation process being too high.
KCNJ3-1 pGHE with a concentration between 34.55ng/uL and 50ng/uL. DNA from Lane 3 on LMC052421A.
This plate indicates that the DNA was circular and that transformation occurred. The colonies are too small and too close together to be used for a maxi-prep. This is due either to the concentration of DNA being too high, or the amount of bacteria being too high.
KCNJ3-2 (assuming in pGHE) with a concentration between 178.78ng/uL and 342.92ng/uL. DNA from Lane 9 on LMC052421A.
This plate indicates the DNA was circular and that transformation occurred. The colonies are too small and too close together to be used for a maxi-prep. This is due either to the concentration of DNA being too high or the number of bacteria being too high.
Negative Control: KCNJ3-1 with a concentration of 69.24ng/uL. DNA from Lane 7 on LMC052421B.
This plate indicates that transformation failed since the linear DNA grew bacteria. This is likely due to the high number of bacteria used during the transformation process, as forty microliters of bacteria is used generally for more complex transformations, such as those with linear DNA.
KCNJ9 pGHE-2 with a concentration of 25ng/uL. DNA was diluted from 327.84g/uL. DNA from Lane 10 on LMC052421A.
This plate indicates that the DNA was circular and that transformation occurred. The colonies were too small and too close together to be used for a maxi-prep. This is either due to the DNA concentration being too high or the number of bacteria being too high.
Negative Control: KCNJ9-1B pGHE Linearized with a concentration of 39.06ng/uL. DNA from Lane 9 on LMC052421A.
This plate indicates that transformation failed as the linear product produced DNA. This is likely due to the number of bacteria used in the transformation process being too high, as forty microliters of bacteria is used for more difficult transformations, such as with linear DNA.
OPRM1-1 pGHE with a concentration of 25ng/uL diluted from 423.73ng/uL . DNA from Box 18.
This plate indicates that the DNA was circular and that transformation occurred. This is likely due to the concentration of DNA being too high or the number of bacteria being too high.
Negative Control OPRM1Purified Aliquot with a concentration of 16.30ng/uL to 25ng/uL. DNA from Lane 2 LMC052421A.
This plate indicates that transformation failed as the linear DNA produced colonies. This is likely due to the number of bacteria used during the transformation process being too high. Forty microliters of bacteria is normally used for difficult transformations, such as those with linear DNA.
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