6/17/21 Nanodrop

eGFP stock:

• Concentration: 5202.5 ng/uL

• 260/280: 2.04

• 260/230: 2.41

eGFP 5/20 6.112 ug/uL

• Concentration: 6913.8 ng/uL

• 260/280: 2.00

• 260/230: 2.28

eGFP 611 ng/uL

• Concentration: 568.2 ng/uL

• 260/280: 2.00

• 260/230: 2.39

eGFP 10/25 3241.7 ng/uL

• Concentration: 2586.7 ng/uL

• 260/280: 1.98

• 260/230: 2.42

eGFP 321.47 ng/uL

• Concentration: 384.2 ng/uL

• 260/280: 1.83

• 260/230: 1.99

Comments:

All of the eGFP tubes are pure, as indicated by the 260/280 results. The eGFP stock, eGFP 5/30 6.112 ug/uL, and eGFP 611 ug/uL tubes have high the highest purity, as a ratio of 1.8 is considered to be pure. 

All of the eGFP tubes are relatively free of contaminants, however, the eGFP stock, eGFP 611 ng/uL, and eGFP 10/25 3241.7 ng/uL tubes have higher values than the 2.0-2.2 range, which could indicate residue on the nanodrop pedestal. 

The eGFP stock tube is assumed to be the stock due to the high volume in the tube and lack of labeling. The high purity of this tube would prove this to be correct.