Embedded Files
Transfection Log
Transfection Log
The transfection log and associated calculators can be found on the Drive. This page is for images of all transfected cells since June 2021, as well as important images of cell health.
6/15/21
6/15/21
1x mRuby, 1.5 DNA lipo, RPMI
7/2/21
7/2/21
Pre-plated 6/28/21
Transfected 6/29/21
eGFP (?) 1x
eGFP (?) 2x
mRuby (pKK) 1x
mRuby (pKK) 2x
RBD-sfGFP (pcDNA3) 1x
7/8/21
7/8/21
Pre-plated 7/5
Transfected 7/6
eGFP (?) 1x
eGFP (?) 2x
eGFP (?) 20x
RBD-sfGFP (pcDNA3) 1x
RBD-sfGFP (pcDNA3) 2x
RBD-sfGFP (mRNA, XmaI) 1x
RBD-sfGFP (mRNA, XmaI) 2x
RBD-sfGFP (mRNA, XhoI) 1x
RBD-sfGFP (mRNA, XhoI) 2x
7/9/21
7/9/21
Pre-plated 7/5
Transfected 7/6
eGFP (?) 1x
eGFP (?) 2x
eGFP (?) 20x
mRuby (pKK) 1x
mRuby (pKK) 2x
mRuby (pKK) 20x
RBD-sfGFP (pcDNA3) 1x
RBD-sfGFP (pcDNA3) 2x
RBD-sfGFP (mRNA, XhoI) 1x
RBD-sfGFP (mRNA, XhoI) 2x
RBD-sfGFP (mRNA, XmaI) 1x
RBD-sfGFP (mRNA, XmaI) 2x
7/16/21
7/16/21
Pre-plated 7/12
Transfected 7/13
Note: Only eGFP 2x, eGFP 5x, and mRuby 2x received 3000. The others received only P3000, as lipofectamine ran out.
Negative control (Opti-MEM only)
Negative control (Opti-MEM with lipofectamine)
eGFP (?) 2x
eGFP (?) 5x
eGFP (?) 10x
mRuby (pKK) 2x
mRuby (pKK) 5x
mRuby (pKK) 10x
7/23/21
7/23/21
Pre-plated 7/19
Transfected 7/20
Negative control (Opti-MEM only)
Negative control (Opti-MEM with lipofectamine)
eGFP (?) 1x
eGFP (?) 2x
eGFP (?) 5x
eGFP (?) 10x
mRuby (pKK) 1x
mRuby (pKK) 2x
mRuby (pKK) 5x
mRuby (pKK) 10x
8/2/21
8/2/21
Pre-plated 7/28
Transfected 7/29
Negative control (Opti-MEM only)
Negative control (Opti-MEM with lipofectamine)
RBD-sfGFP (pcDNA3) 2x
eGFP (?) 1x (stock)
eGFP (?) 2x (stock)
eGFP (?) 5x (stock)
eGFP (?) 1x (dilute)
eGFP (?) 2x (dilute)
eGFP (?) 5x (dilute)
eGFP (?) 1x (stock, lipofectamine 3000 only)
eGFP (?) 2x (stock, lipofectamine 3000 only)
eGFP (?) 5x (1x lipofectamine)
9/24/21
9/24/21
Pre-plated 9/20
Transfected 9/21
Negative control (Opti-MEM without cells)
Negative control (Opti-MEM only)
Negative control (Opti-MEM with lipofectamine)
eGFP (pEGFP-N1) 1x
eGFP (pEGFP-N1) 2x
eGFP (pEGFP-N1) 3x
mRuby (pcDNA3) 1x
mRuby (pcDNA3) 2x
mRuby (pcDNA3) 3x
RBD-sfGFP (mRNA) 1x
RBD-sfGFP (mRNA) 2x
RBD-sfGFP (mRNA) 3x
10/8/21
10/8/21
Pre-plated 10/4
Transfected 10/5
Negative control (Opti-MEM only)
Negative control (Opti-MEM with lipofectamine)
Negative control (Opti-MEM without cells)
eGFP (pEGFP-N1) 1x
eGFP (pEGFP-N1) 2x
eGFP (pEGFP-N1) 3x
RBD-sfGFP (XhoI mPSI, mRNA) 1x
RBD-sfGFP (XhoI mPSI, mRNA) 2x
RBD-sfGFP (XhoI mPSI, mRNA) 3x
RBD-sfGFP (XhoI, S) 1x
RBD-sfGFP (XhoI, S) 2x
RBD-sfGFP (XhoI, S) 3x
RBD-sfGFP (XhoI, L) 1x
RBD-sfGFP (XhoI, L) 2x
RBD-sfGFP (XhoI, L) 3x
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