P-97 Pipette Puller

Press Program# and ENTER (ENTR)

Press CLEAR (CLR) and #1

Enter the following values

Press CLEAR (CLR) and #0 to change pressure

(See the display and Press # corresponding to pressure)

In Dr Ostrowski's lab, use Program #51 for oocyte injection micropipettes, #55 for Channel 1 micropipettes, #56 for Channel 2 micropipettes.

Use Heat = 545, Velocity = 25, Time = 250 for Program #55 in Dr Ostrowski's lab. You should get Channel #1 micropipettes with straight tips.

Use Heat = 530, Velocity = 16, Time = 250 for Program #56 (Channel #2) in Dr Ostrowski's lab.

Please use the reset button when you want to move from one program to another in the P-97 Micropipette Puller in Dr Ostrowski's lab. If you are the last person to use it for the day, please put the cover on it when you are done.