Embedded Files
6/08/21
6/08/21
The plasmid was retrieved from the mail room as a bacterial stab and stored in Fridge B.
6/09/21
6/09/21
Two plates of hACE2_pcDNA3.1 were streaked and isolated for colonies by HS. The plates were placed in the incubator at 37C overnight.
6/10/21
6/10/21
Sequencing primers were designed and ordered for hACE2_pcDNA3.1
hACE2_pcDNA3.1 was inoculated by HS at 5pm.
6/11/21
6/11/21
hACE2_pcDN3.1-1 and hACE2_pcDN3.1-2 was midiprepped at 8am by HS. After the 100% ethanol step (step 8) two very very small pellets formed so .80mL and 1.15mL of sodium acetate buffer was added to hACE2_pcDN3.1-1 and 2, respectively. The tubes were inverted 10 times and left overnight.
6/12/21
6/12/21
hACE2_pcDNA3.1 were centrifuged at 10,000g for 3 minutes by LC. Completed the last ethanol step and left the pellet to dry over 2 nights.
6/14/21
6/14/21
Resuspended the pellet in 20uL of MBG Water by HS. Ran on a gel by HS. Both midipreps failed.
6/15/21
6/15/21
Maxiprepped by LC. No large pellets formed after the 100% ethanol step, so 3.3mL and 3.5mL of sodium acetate buffer was added to hACE2_pcDNA3.1-1 and 2 respectively. The solutions are sitting overnight.
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