Using the Large Autoclave

Video

Location

1. Get the pink key from above the incubator (Ingrid). 

2. Make sure all bottles/flasks have autoclave tape (top drawer in plastic filing cabinet under the balance). Put a piece of tape on the plastic bin too. 

3. Make sure all bottles are not completely screwed tight. Leave the cap on and a small amount of room so that gas can escape.

4. Go to the Basement floor, off the elevator, take a left down the hall. The room will be at the very end of the hall on the left side.

5. The big autoclave is in the corner. 

Operation

1. Gently open the door, it can slam. Place the whole plastic bin in there, and gently close the door.

2. If you have any liquids at all, pick the liquid cycle. 

3. If you don't have liquids, pick the gravity cycle. 

4. It'll give a time, but add around 20 minutes to that because it needs time to cool down and depressurize. 

5. Once your timer is up, bring the key, a cart, and the large orange gloves back down to the basement.

6. Before opening the door, make sure both the chamber and jacket pressures are zero. 

7. The autoclaved glassware will be HOT!!! Leave the door open.