I + I

For x mL of I+I needed (e.g. 250 mL), you will need the following:

500uM:

·       x mL Frog Ringer’s (250mL)

      x/2 uL Isop (250/2 = 125 uL)

       x uL IBMX (250 uL)

1mM:

·       x mL Frog Ringer’s (250mL)

·       x/2 uL Isop (250/2 = 125 uL)

·       2x uL IBMX (250*2 = 500 uL)