Remove cells from -80 freezer
Thaw in warm water bath until a small chunk of ice is left in the vial
Add small amount of media to vial so as to dilute DMSO
Move vial contents to 15 ml centrifuge tube and further add media until reaching 10 ml total
Centrifuge at 1000 rpm for 2 min
Aspirate
Resuspend in proper media and incubate