Thawing Cells

    1. Remove cells from -80 freezer

    2. Thaw in warm water bath until a small chunk of ice is left in the vial

    3. Add small amount of media to vial so as to dilute DMSO

    4. Move vial contents to 15 ml centrifuge tube and further add media until reaching 10 ml total

    5. Centrifuge at 1000 rpm for 2 min

    6. Aspirate

    7. Resuspend in proper media and incubate