Microfluidic Sorting

[Motivation/Question] How can you separate differentiated cells from undifferentiated mouse embryonic stem cells (mESCs)?

[Current Status]: Active sealing method (ASM) for reversible bonding technique (H.Bang, W.G.Lee et al., JMM, 2006; W.G.Lee et al., MST, 2008)

[Next Step]: To enrich undifferentiated mESCs in a microfluidic format

[Research Topic]: Microfluidic enrichment of undifferentiated mESCs

[Table of Contents]

1. Introduction (Reference)

Rohit Karnik's Nano Letters (2009) - p selectin based microfluidic sorting of HL60 using cell rolling mechanism

2. Materials and Methods

2.0. Device design and fabrication

2.1. Oct4 ESCs

2.2. Local surface treatment by ASM

2.3. RT-PCR

2.4. Immunostaining

2.5. FACS

2.6. Cell viability

2.7. Statistical analysis (Student paired t-test)

2.8. Functional analysis (if necessary)

3. Resutls

4. Discussion

5. Conclusions