[Motivation/Question] How can you separate differentiated cells from undifferentiated mouse embryonic stem cells (mESCs)?
[Current Status]: Active sealing method (ASM) for reversible bonding technique (H.Bang, W.G.Lee et al., JMM, 2006; W.G.Lee et al., MST, 2008)
[Next Step]: To enrich undifferentiated mESCs in a microfluidic format
[Research Topic]: Microfluidic enrichment of undifferentiated mESCs
[Table of Contents]
1. Introduction (Reference)
Rohit Karnik's Nano Letters (2009) - p selectin based microfluidic sorting of HL60 using cell rolling mechanism
2. Materials and Methods
2.0. Device design and fabrication
2.1. Oct4 ESCs
2.2. Local surface treatment by ASM
2.3. RT-PCR
2.4. Immunostaining
2.5. FACS
2.6. Cell viability
2.7. Statistical analysis (Student paired t-test)
2.8. Functional analysis (if necessary)
3. Resutls
4. Discussion
5. Conclusions