Projects Micropatterned for
Internal Anal Sphincter Tissue Engineering

[Patent]

Title:

Three dimensional bioengineered smooth muscle tissue and sphincters and methods therefor

Document Type and Number:

United States Patent 7368279

Abstract:

This invention relates to a three dimensional bioengineered sphincter such as a bioengineered internal anal sphincter (IAS) and three-dimensional physiological models of smooth muscle cells. The invention also related to methods for generating a bioengineered sphincter and smooth muscle rings in culture. In addition, the invention provides for methods of using a bioengineered sphincter or bioengineered smooth muscle ring to identify agents that modify contractile force and relaxation in smooth muscle cells.

http://www.freepatentsonline.com/7368279.html

[Journal]

Main reference:

Hecker, L., K. Baar, et al. (2005). "Development of a three-dimensional physiological model of the internal anal sphincter bioengineered in vitro from isolated smooth muscle cells." Am J Physiol Gastrointest Liver Physiol 289(2): G188-96. http://ajpgi.physiology.org/cgi/content/full/289/2/G188

Kang, S. B., H. N. Lee, et al. (2008). "Sphincter contractility after muscle-derived stem cells autograft into the cryoinjured anal sphincters of rats." Dis Colon Rectum 51(9): 1367-73.

Furuta, A., R. J. Jankowski, et al. (2007). "The promise of stem cell therapy to restore urethral sphincter function." Curr Urol Rep 8(5): 373-8.

Reconstruction:

Chen, Y. L. (1983). "[Reconstruction of the anal internal sphincter using smooth muscle of the colon: report of 5 cases]." Zhonghua Wai Ke Za Zhi 21(5): 260-1.

Sato, S. and S. Nakamura (1970). "[Application of construction of an artificial anus through the sphincter for the repair of urethrorectal fistula]." Shujutsu 24(4): 413-8

De Carvalho Pinto, V. A. and P. Campos Nogueira (1955). "Construction of the sphincter of the anus and restoration of anal continence by transplanting the gracilis muscle." J Pediatr 46(4): 442-6.

Artifical sphincter:

Benoist, S., Y. Panis, et al. (2005). "Artificial sphincter with colonic reservoir for severe anal incontinence because of imperforate anus and short-bowel syndrome: report of a case." Dis Colon Rectum 48(10): 1978-82.

Szinicz, G. (1980). "A new implantable sphincter prosthesis for artificial anus." Int J Artif Organs 3(6): 358-62.

Bondar, G. V. and A. I. Fefelov (1972). "[Creation of a functioning artificial sphincter during the formation of a preternatural anus]." Klin Khir 5: 52-5.

<Animal Model>

Fan, Y. P., R. N. Puri, et al. (2002). "Animal model for angiotensin II effects in the internal anal sphincter smooth muscle: mechanism of action." Am J Physiol Gastrointest Liver Physiol 282(3): G461-9.

Vinograd, I., M. Hanani, et al. (1985). "Animal model for the study of internal anal sphincter activity." Eur Surg Res 17(4): 259-63.

Vinograd, I., M. Hanani, et al. (1984). "[Neuropharmacological study of the internal anal sphincter in an animal model]." Harefuah 107(9): 221-4.

Related stem cells research:

Tian, C., Y. Lu, et al. (2008). "Differentiation of murine embryonic stem cells in skeletal muscles of mice." Cell Transplant 17(3): 325-35.

Cerletti, M., S. Jurga, et al. (2008). "Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles." Cell 134(1): 37-47.

Interanl ananl sphincter:

<mechanical properties>

Glavind, E. B., A. Forman, et al. (1993). "Mechanical properties of isolated smooth muscle from human rectum and internal anal sphincter." Am J Physiol 265(4 Pt 1): G792-8.

<contraction regulation>

Bitar, K. N., C. Hillemeier, et al. (1991). "Regulation of smooth muscle contraction in rabbit internal anal sphincter by protein kinase C and Ins(1,4,5)P3." Am J Physiol 260(4 Pt 1): G537-42.

<differential regulation>

Bitar, K. N., C. Hillemeier, et al. (1990). "Differential regulation of smooth muscle contraction in rabbit internal anal sphincter by substance P and bombesin." Life Sci 47(26): 2429-34.

Rattan, S. (2005). "The internal anal sphincter: regulation of smooth muscle tone and relaxation." Neurogastroenterol Motil 17 Suppl 1: 50-9.

Abbasakoor, F., A. R. Morgan, et al. (1998). "Internal anal sphincter repair." Int J Colorectal Dis 13(1): 53.

Leroi, A. M., M. A. Kamm, et al. (1997). "Internal anal sphincter repair." Int J Colorectal Dis 12(4): 243-5.

Bourdelat, D., J. P. Barbet, et al. (1990). "The morphological differentiation of the internal sphincter muscle of the anus in the human embryo and fetus." Surg Radiol Anat 12(2): 151-2.

Leutenegger, F. (1969). "[Studies of the internal sphincter anus muscle for ganglia cells]." Schweiz Med Wochenschr 99(40): 1431-2.

Neuro-effector transmission

Kubota, M., S. Suita, et al. (1998). "Membrane properties and the neuro-effector transmission of smooth muscle cells in the canine internal anal sphincter." J Smooth Muscle Res34(4): 173-84.

Replacing

Laichman, S. and V. Skacel (1986). "The internal obturator muscles functioning as the neosphincter of the anus (I). An experimental study of the muscles used for replacing the sphincter of the anus." Acta Univ Palacki Olomuc Fac Med 113: 271-9.

Electric Stimulation

Kaneko, S., Y. C. Park, et al. (1983). "[Urodynamics. IX. Therapy of urge incontinence by electric stimulation of anus sphincter]." Nippon Hinyokika Gakkai Zasshi 74(8): 1339-45.

External rectal sphincter

Suvorova, G. N. (2002). "[Regeneration of striated muscular tissue of the external rectal sphincter in the rat]." Morfologiia 121(1): 89-91.

Astiz, J. M. and M. Sibolich (1971). "[Sphincter apparatus of the anus]." Prensa Med Argent 58(19): 968-72.