Material :
- Neutral Buffered Formalin 10%
- 80%EtOH
- 95%EtOH
- 100%EtOH
- Xylene
- Paraffin
Procedure :
Put the cassettes with the samples in the following solutions :
1) 10% neutral buffered formalin for 1 hour
2) 10% neutral buffered formalin for 1 hour
3) 70% EtOH for1 hour (or overnight)
4) 95% EtOH for 1 hour
5) 95% EtOH for 1 hour
6) 100%EtOH for 1 hour
7) 100%EtOH for 1 hour
8) 50/50 100%EtOH/Xylene for 1 hour
9) Xylene for 1 hour
10) Xylene for 1 hour
11) Paraffin for 2 hours in the oven at 60 degrees Celsius
12) Paraffin overnight in the oven at 60 degrees Celsius
Now, we can embed the sample with the embedding machine:
1. Turn on the machine and make sure that there is enough paraffin in the two paraffin container.
2. Poor the metal mold with paraffin
3. Put the sample into the paraffin
4. Cover the metal mold with the cassette
5. Add paraffin to cover the cassette
6. Let the sample solidify on the cold plate