Acryloyl-PEG-peptide preparation

Materials:

Peptides conjugated to monoacrylated PEG

acryloyl-PEG-n-hydroxysuccinimide (3400 Da; Nektar Therapeutics, Birmingham, AL)

50 mM sodium bicarbonate buffer

Procedure:

1) Peptides were conjugated to monoacrylated PEG as previously described

2) Briefly, peptides were combined with acryloyl-PEG-n-hydroxysuccinimide in 50 mM sodium bicarbonate buffer (pH 8.5)

3) Reaction allowed to take place at room temperature for 2 h.

4) The resulting acryloyl-PEG-peptides were lyophilized and stored at -20°C until use.