Materials:
Peptides conjugated to monoacrylated PEG
acryloyl-PEG-n-hydroxysuccinimide (3400 Da; Nektar Therapeutics, Birmingham, AL)
50 mM sodium bicarbonate buffer
Procedure:
1) Peptides were conjugated to monoacrylated PEG as previously described
2) Briefly, peptides were combined with acryloyl-PEG-n-hydroxysuccinimide in 50 mM sodium bicarbonate buffer (pH 8.5)
3) Reaction allowed to take place at room temperature for 2 h.
4) The resulting acryloyl-PEG-peptides were lyophilized and stored at -20°C until use.