1) Immerse coverslip with fibronectin solution (20-200 ng/mL concentration)
2) Incubate for at least 15 min
3) Remove old media from cells
4) Add enough trypsin (1X-10X concentration) to cover bottom of flask
5) Wait a few minutes until cells no longer adhere to flask (optional to place in incubator or to tap flask gently)
6) Dilute with PBS and pellet cells
7) Remove supernatant and resuspend cell pellet in desired amount of fresh media
8) Remove fibronectin solution from coverslips
9) Tether cells to coverslip by incubating cells with fibronectin coated coverslips for several hours (~2 hours at least)