basic microfluidics supplies
SILICON WAFERS
Silicon Sense, Inc.
603-891-4248
3" wafers, orientation <100>, thickness: 15+-2 mil, 25 wafers / box
type / dopant: N/Phos, resistivity: 1-10, grade: test
TUBING
- PTFE #30 AWG Thin Wall Tubing Natural, ID=0.012”, OD=0.030”, 100 ft
Provider: Cole Parmer; 800-323-4340, item #: 06417-11 (this tubing is
commonly inserted directly into the device, without a connector)
- Tygon microbore tubing, ID=0.02”, OD=0.06”, 100 ft
Provider: Cole Parmer; item #: EW-06418-02 (I use this tubing with
metal connectors, see below)
- small metal tubing (metal posts): RW 23, type 316L (length = 0.3”), ID=0.013”,
OD=0.025”, 100 pieces
Provider: New England Small Tubing Comp., Litchfield, NH; 603-429-1600
(fits Tygon tubing, but not PTFE tubing)
NEEDLES & STUBS
- Luer Stub Adapter, OD=0.65 mm (for Tygon tubing)
Provider: Becton Dickinson, Franklin Lakes, NJ
- Luer Stub 27 ½, (for PTFE tubing)
Provider: Becton Dickinson, Franklin Lakes, NJ
commonly, 1/2" or 1" is sufficient, and I prefer stubs to needles
GASTIGHT SYRINGES
recommended for any use, especially at low flow rates (up to ~200 ul/hr)
also recommended for any gradient applications
I don't recommend using plastic syringes, because they're not gastight (that is, you can get air bubbles forming in the syringe and tubing). If you can use a glass syringe for cell applications, try to keep the cells confined to the tubing.
- 500 ul, item #: 81220
- 50 ul, item #: 80920
- 250 ul, item #: 81120
- 5 ml, item # 81520
Provider: Hamilton Company, Reno, NV; 800-648-5950 (these come with a glass base, so they tend to break)
even better: SGE, as they come with a metal base
http://www.sge.com/products/1ml-to-100ml-gastightsyringes
- 1 ml, item #: 008025
- 2.5ml, item #: 008425
- 5 ml, item #: 008762
SYRINGE PUMP
I recommend Harvard PHD 22/2000 Pump (infuse + withdraw, doesn't have to be programmable, goes beyond 3000ul/hr). It's the most precise syringe pump I've worked with (highest precision ~0.01%). Make sure you match the syringe to the applied flow rate (i.e. don't use 5 ml syringe for a 100ul/hr flow rate, but use 500ul or 1 ml syringe).
ELASTOMER
- PDMS: Sylgard 184
http://www.ellsworth.com/sylgard.html?gclid=CPPTmLKw-KICFSI55QodRRdFlQ
2-part elastomer, I recommend the 0.5 kg kit
- RTV: from GE Chemicals
http://www.mgchemicals.com/products/615.html
(bad for protein, so I assume bad for cells, but in general great to work with, especially for multiplayer microfluidic devices)
preferred distributor is Hisco
LABELING TAPE
salmon: Fisher Scientific, Fisherbrand Cat no: 15959
yellow: Fisher Scientific, Fisherbrand Cat no: 1590130B
TWEEZERS
Dumont #3 tweezers, stainless steel
Cat no: 72691-D