Begin Paper Summary Template
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DATE CREATED: Jan 19 2009
ACTION PLAN
PROBLEMS IDENTIFIED (PI) / OUTSIDE SKILL REQUIRED (OSR) / RESOLVED (R)
Problem (and date)
Action
Status
F) RESULTS
Biocompatibility of fibrin gels
- Figure : Contact angle of candidate hydrogels : fibrin, agarose, gelatin
Optimization of printing dot size
- Figure : glass tip size and flow rate, without cells
- Figure : Flow rate and dot size, eqn and evaluated
Channel formation in hydrogels
- Figure : Branched vascular structure
Multiple cell type patterning with different functions
- Figure : Fluorescence labelled
G) DISCUSSION
H) ANTICIPATED FIGURES FOR PAPER or when you have data, FIGURES FOR PAPER
Figure 1. Schematic of cell printing
Figure 2. Contact angle of candidate hydrogels : fibrin, agarose, gelatin
Figure 3. Glass tip size and flow rate, without cells
Figure 4. Flow rate and dot size, eqn and evaluated
Figure 5. Branched vascular structure
Figure 6. Fluorescence labelled
I) FUTURE DIRECTIONS
Make liver structure to
J) LITERATURE
PAPER TITLE : 3D Cell printed liver tissue engineering with branched microvascular structure: Communication to Biomaterials
A) BACKGROUND
In tissue engineering, the vascularization or angiogenesis of the tissue in the engineered 3D cell architecture is one of challenging field.
Until now, majority of research reports about blood vessel includes tissue engineered vascular structures in relatively large scale, and angiogenesis with growth factors in small scale.
Some challenges were done with microvascular structure by microfluidic system with different vascular cells in the tubular structures[SH Lee, under publication].
However, the vascular structure of real tissues are complex and has several branches and even more small vessels of capillary. Most of hormon, neurients, ions and oxygen exchange occurs in the tissue close to capillaries.
And controlled approach of branched microvascular structure with endothelial cells.
Cell printing is one of promising field in tissue engineering filed, especially for complex and multiple cell type organ regeneration.
Complex vascular structure
B) HYPOTHESIS
Single dots of printed cells can generate connected structure and cells can survive after dissolving the matrix.
C) SPECIFIC AIMS
AIM 1 - Print cells with desired pattern (MIT or something)
AIM 2 - Print branched vascular structures with cell printer
D) GENERAL EXPERIMENTAL APPROACH (Materials and Methods)
Cell preparation
Printing cells on the fibrin gels
Biocompatibility of printed cells on fibrin gel
Formation of branched vascular structures
Immunofluorescence
SEM of vascular structure
E) PITFALLS AND ALTERNATIVES