7-10-09

July 10, 2009

Won Gu: absent

Masoud: absent

New member: Cheong Hoon Kwon

Dr. Sim:

· Introduction of the progress of the project

· Get the basal differentiation ratio of bone lineage

· Macro stimulation and micro stimulation

· Promising results on the expression of the mesoderm mark (bone and cartilage linear differentiation )using Q PCR brachyury and Type I collagen increasing in the macro stimulation

· Propose the outline and expected figures for the 3D gelatin encapsulation EB

· Problem with micro scale test results (cell death or contamination)

Suggestion:

· Add Collagen control to the project to compare with the gelatin

· Be careful of the time line to make sure finish the projects in time

Jiankang

· Performing experiments for revising the previous paper, which shows that

· Progress on the current project to build composite materials using microfluidic devices

· Try to solve the thin-skin problem of the gelatin and chitosan by adding a top layer of water.

· Show cell gradient on the gelatin and chitosan

· Chitosan shows better cell attachment than gelatin, try to figure out the problems

Future plan:

Change chitosan to Hyaluronic acid

Characterize the materials using SEM and XPS.

Investigate the cell behaviors on the porous structures

Matt and Du

Update the progress of the gradient paper

Will have more progress in the next week.