Yu-Shik Hwang
Meeting Summary Page - Please complete after each subgroup meeting.
Research Interests:
Control of Embryonic Stem Cell/Induced Pluripotent Stem Cell Differentiation
Tissue Engineering
Cell Biology
BioMEMS
Bioprocessing
Experience & Skills:
Experience
Ph.D Degree
School: Imperial College London, UK.
Period: Sep.2003 –Apr. 2008
Major: Chemical Engineering, Tissue Engineering & Regenerative Medicine
Thesis: In vitro osteogenic/chondrogenic differentiation of embryonic stem cells for bone/cartilage tissue engineering
Research: Embryonic stem cell based skeletal tissue engineering
Master Degree
School: Joint Graduate Program : Yonsei University, Seoul, Korea., Korea Institute of Science and Technology (KIST), Seoul, Korea
Period: 1998 – 2000.
Major: Medical Engineering (Yonsei Univ) & Biomaterials (KIST)
Thesis: Ozone induced type I atelocollagen grafting onto polymer and cellular activity related to grafted collagen
Bachelor Degree
School: Yonsei University, Seoul, Korea
Period: 1993 – 1998.
Major: Biochemistry
Thesis: Site directed mutagenesis of malonyl-CoA decarboxylase
Work Experience I
Institution: Department of Chemical Engineering and Chemical Technology Imperial College London, UK
Period: Jan. 2006 – Apr. 2008
Role: Research Assistant (Funded by DTI)
- DTI Project “Developing Practical Applications of Stem Cell Technology”
Work Experience II
Institution: Medical Technology and Quality Evaluation Center (MTEC) Yonsei Medical Research Center, Seoul, Korea
Period: Mar. 2003 – Sep.2003
Role: Senior Researcher
- Biocompatibility evaluation of Medical Devices for commercialization.
Work Experience III
Regnemed Inc (Bio-venture company), Seoul, Korea
Period: Sep.2001 – Feb. 2003
Role: Senior Researcher
- Managing research team: research and develop collagen based biomaterials for tissue regeneration
Skills
Softlithography based Microfabrications
Humans and murine embryonic stem cell expansion and differentiation
Umbilical Cord Blood 34+ Stem Cell isolation and expansion
Primary mammalian cell culture from tissue biopsy (esophagus, Liver)
Various techniques relating with cell proliferation, differentiation and migration
3D culture using a HARV and Perfused bioreactor culture system
Fabrication of porous 3 D scaffolds using polymers (PU, PLGA, PLLA) and natural biomaterials (Collagen, HA, Hydrogels)
Characterization of surface modified polymer (DCA, EDS, SEM, FTIR)
MACS and FACS analysis
Biocompatibility test such as cytotoxicity, genotoxicity, irritation and sensitization
Biological assay for the cellular interaction with extracellular matrix (ELISA, SDS, various functional assays)
Extraction and purification of bio-macromolecules (Type I, II collagen, GAG)
Microbial susceptibility test such as MIC and MCC
Implantation of biomaterials into small animal: In vivo test
Conventional staining, histological staining, and immuno-cytological and-histological staining
Electrophoresis and western blot
Molecular works such as RT-PCR, real time RT-PCR
Project Pages:
(click link on how to set up your project pages - will open in a new window)
1. EB size mediated Cardiomyogenesis and Vasculogenesis