4-30-09

4-30-09

Won Gu:

Left for the past week, so do not have many updates on the project.

Briefly introduce his project. For the future plan, microfluidic mixer,

· Develop and characterize the mixer (1 month), which is engineering-oriented paper.

Performance of the device, feasibility of generating different combination, individual loading of the microwell.

· Stem cell application using this device, which is biology-oriented paper.

Matt and Du:

Show the theoretical work and experimental work of the gradient project.

Fitting to Delta vs. t theory curves

Optimization the gradient growth v.s. the peclet number and channel shape.

Show the cell and microbeads gradient.

Suggestion: try to see how the oscillation improve the gradient formation of bead and cells

Future plan:

1. Choose good exposure time (I think 0.8s, but we need to check), update min (empty), max, gradients for various exposure times

2. Update the exposure profile

3. photo bleaching: 1 pic every 5 sec, 1.0s exposure (or whatever was used in all our expts), repeat 20 times, process images -> produce excel, send to matt

4. Repeat expt but take pics every 20s

5. How to get the full profile of the gradient