2.6. Immunostaining
2.7. Statistical analysis (Student paired t-test)
2.8. Functional analysis (if necessary)
* Dr. Sanghyuk Park could perform these in his lab at their expense if Prof. Ali approved this outline (including collaboration with Kaplan group, Tufts Univ.).
* Dr. Sanghyuk Park is one of my Korean colleagues as well as one of postdocs in Kaplan group.
3. Resutls
4. Discussion
5. Conclusions
2.5. CLSM*
[Current Status]: A hollow sphere (HS) soft lithography was recently developed.
[Next Step]: To confirm if cystic embryoid bodies (EBs) can be made in the HSs.
[Research Topic]: Earlier formation of cystic EBs in long-term hanging drops (HDs)
[Table of Contents]
1. Introduction
2. Materials and Methods
2.1. R1 ESCs
2.2a. RT-PCR
2.2b. qPCR*
2.3. ELISA*
2.4. SEM*