Hollow Spheres (expanded)

2.6. Immunostaining

2.7. Statistical analysis (Student paired t-test)

2.8. Functional analysis (if necessary)

* Dr. Sanghyuk Park could perform these in his lab at their expense if Prof. Ali approved this outline (including collaboration with Kaplan group, Tufts Univ.).

* Dr. Sanghyuk Park is one of my Korean colleagues as well as one of postdocs in Kaplan group.

3. Resutls

4. Discussion

5. Conclusions

2.5. CLSM*

[Current Status]: A hollow sphere (HS) soft lithography was recently developed.

[Next Step]: To confirm if cystic embryoid bodies (EBs) can be made in the HSs.

[Research Topic]: Earlier formation of cystic EBs in long-term hanging drops (HDs)

[Table of Contents]

1. Introduction

2. Materials and Methods

2.1. R1 ESCs

2.2a. RT-PCR

2.2b. qPCR*

2.3. ELISA*

2.4. SEM*