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Meeting Date: Jan. 6th, 2009
Summary: 1.3T3 cell viability tested in sealed microwells: operation window is 6hr w/ ~100 cells/well. 2. Dye release speed tested: direct printing, ~2 min; encapsulated in PEG 258, days.
Action Plan:1. Test different materials/conditions for controlled release. 2. test alcohol induced cell death to demonstrate system function.
Progress on issues from last meeting: 3T3 cell works for quantification purpose.
Meeting Date: Dec 19, 2008
Summary: 1.Confirmed previous experiments of effective microwell sealing, good for up to 3 days. 2. Viability test of HepG2 cells in sealed PDMS microwells shows our operating window is within 2hrs after sealing.
Action Plan:Use 3T3 cells to quantify viability data. Start print chemical arrays and work on alignment of array with microwells; characterizing chemical release speed from array surface into microwell; investigate possible ways to control release speed.
Progress on issues from last meeting: HepG2 aggregates issue in this report, to be solved by use of 3T3.
Meeting Date: Dec 11, 2008
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Meeting Date: Dec 2, 2008
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Meeting Date October 28 2008
Summary Array slide trays not working properly, solution - shut down at night.
small irregular spots, lot of time to cover well, increase spot size and change concentration.
Put cells in PEG micro-wells to grow into aggregates.
PURPOSE ? Why testing ? Define problem. Why cell death ? Other problems ? Leeching ? Poor-cross linking or gel fabrication ? Any data on cell death ? Controls etc ?
What is going to be your main project - Define for next meeting.
Ali's notes - Working on A6, why printing PEG, come up with your own project, other collaborations on the side.