Hydrogel fabrication : Alginate, Agarose, Fibrin, PEG, HA-MA, Gelatin, Collagen
Fiber generation with microfluidic chip
Primary culture of neural cells : rat
Primary culture of cardiomyocytes
Microfluidic gradient generation & application to cell migration
PDMS work
Immunocytochemistry
SEM & Confocal microscope
Project Pages: (Help)
1) PEGDA based soft lithography
2) Ice patterning of cell-laden hydrogels
3) Cell printing : Liver tissue engineering with branched microvascular structure
4) Cardiac tissue regeneration with fiber template
5) Liver tissue engineering with cell laden porous gel
6) [Closed] Microengneered microvascular network for 3D tissue construction
7) [Finished] Grant : Microengineered 3D liver tissue engineering and drug screening
changmo@gmail.com
Research Interests:
Microengineered tissue engineering
Tissue engineering in porous hydrogel
Cardiomyocyte based heart tissue regeneration
Experience & Skills:
Meeting Summary (Help)
Meeting Date: Jan. 06, 2009
Summary:
Liver TE: Finished controlling gelatin microbeads, microsphere size between 150-300 micron
3D microstructure of HA-IPN (Onur project): Synthesize HA, preparing materials
Cardiac cell fiber: tried with cardiomyocytes(CM) incorporation in alginate microfiber, cells did not spread in the gel
Culture CM and 3T3 in fibrin gel, CM do not spread, 3T3 spread : CM needs stiff gel matrix, ref 2005 Science 10kPa=> Gelatin crosslinking (Dr. Hojae)
Action Plan:
Liver TE : Ca++ ion incorporation in the microspheres to make alginate homogeneous gelation
Preliminary study with porous cell laden gel and albumin assay
3D Microfabrication(Onur) : Cell compatibility evaluation of cells \
Cardiac TE : Cell culture on different stiffness gelatin gels
Progress on issues from last meeting:
Meeting Date: October 28 2008
Summary: Changmo - staining cells for viability current method does not work well. Artifact of cell viability. Problems with diffusion of dyes ? Review literature.
Gelling alginate/agarose
Action Plan:Paper outline
Meeting Date - October 19 2009
Summary - Structure talk introduction, background etc.
Liver tissue engineering. Ice patterning. How does this differ from other work ? Advantages ? Disadvantages ? Practicality ?
Define issues, problems that people have not yet solved what is the impact ?