4-16-09
Masoud and Bonggeun:
· Cell-patterning in the side-well groove. 90oc , 60oc, 30oc. Simulation of the flow scream in the microgroove channel. Cells won’t go into the 60 degree channel (flow pattern is not going into the groove).
· Using the device for cell sorting application, 10um 5um microbeads. . Smaller ones will go deeper inside the grooves while large beads stay in the center. Potentially pattern two-type cells, bacteria cell separation.
· Numerical simulation
· Single cell docking
Suggestion:
· How to solve cell culture problem since the wall is non-adhesive to the cells.
· How to you define the sorting efficiency? How to improve the sorting effect
· Harvest the specific cell from the two cell population?
Dr. Sim
· Improve the cell loading into the micro-well after BSA coating
· Optimization of the cell –loading channel to prevent cell-clogging problem in the channel
Suggestion:
· Adding washing step after BSA treatment to prevent cell clogging
· To achieve Air compression using the 6-layer devices
Jiankang
· SEM image to characterize the air-dried and freeze-dried hydrogel gradient to show different thickness and porosity.
· RGD-modified PEG gradient to mediate the cell adhesion and spreading.
· Mechanical gradient of the material stiffness to regulate the cell morphology
· Planning a new project by encapsulating the cells in the materials
· Control cell-cell interaction spatially and temporally by studying the HUVEC and cardiomyocyte
Suggestion:
· Improve the length of the gradient
· Change to a new method back-and-forth pumping to improve the gradient length
· Encapsulation of the cardiac cells in the HA/collagen gels
· Why is it important to varying the ratios of the two types of cells? 3D environment
· Try to make outlines for the new project
· If sell the paper for High-through system, need to find the right material in which vascularization can occur
· Is it possible to sell the paper for engineering bio-mimetic cartilage-bone-interface
Seda
· Introduction of the outline of the cell-based biosensor
Suggestion:
· Try to incorporate the Microwell sandwich assay and CCD chip to the detection part.
· Nano-particle patterning to concentrate the Troponin I
· Reversible sealing to localize the treatment