4-16-09

Masoud and Bonggeun:

· Cell-patterning in the side-well groove. 90oc , 60oc, 30oc. Simulation of the flow scream in the microgroove channel. Cells won’t go into the 60 degree channel (flow pattern is not going into the groove).

· Using the device for cell sorting application, 10um 5um microbeads. . Smaller ones will go deeper inside the grooves while large beads stay in the center. Potentially pattern two-type cells, bacteria cell separation.

· Numerical simulation

· Single cell docking

Suggestion:

· How to solve cell culture problem since the wall is non-adhesive to the cells.

· How to you define the sorting efficiency? How to improve the sorting effect

· Harvest the specific cell from the two cell population?

Dr. Sim

· Improve the cell loading into the micro-well after BSA coating

· Optimization of the cell –loading channel to prevent cell-clogging problem in the channel

Suggestion:

· Adding washing step after BSA treatment to prevent cell clogging

· To achieve Air compression using the 6-layer devices

Jiankang

· SEM image to characterize the air-dried and freeze-dried hydrogel gradient to show different thickness and porosity.

· RGD-modified PEG gradient to mediate the cell adhesion and spreading.

· Mechanical gradient of the material stiffness to regulate the cell morphology

· Planning a new project by encapsulating the cells in the materials

· Control cell-cell interaction spatially and temporally by studying the HUVEC and cardiomyocyte

Suggestion:

· Improve the length of the gradient

· Change to a new method back-and-forth pumping to improve the gradient length

· Encapsulation of the cardiac cells in the HA/collagen gels

· Why is it important to varying the ratios of the two types of cells? 3D environment

· Try to make outlines for the new project

· If sell the paper for High-through system, need to find the right material in which vascularization can occur

· Is it possible to sell the paper for engineering bio-mimetic cartilage-bone-interface

Seda

· Introduction of the outline of the cell-based biosensor

Suggestion:

· Try to incorporate the Microwell sandwich assay and CCD chip to the detection part.

· Nano-particle patterning to concentrate the Troponin I

· Reversible sealing to localize the treatment