Fabrication of gradient hydrogels to guide cell behavior

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DATE CREATED: Jan 7, 2009

ACTION PLAN

PROBLEMS IDENTIFIED (PI) / OUTSIDE SKILL REQUIRED (OSR) / RESOLVED (R)

PAPER TITLE

A) Background.

B) Specific Aims:

C) General Experimental Approach (Design etc):

D) Design Pitfalls and alternatives

E) Potential Figures:

F) Future Directions:

A) BACKGROUND:

Fabrication of stable long-distance gradient for biological study.

B) SPECIFIC AIMS

AIM 1 - modulate cell morphology and migration

AIM 2 -

C) GENERAL EXPERIMENTAL APPROACH

Microfluidics, Cell culture

D) DESIGN PITFALLS AND ALTERNATIVES

E) POTENTIAL FIGURES FOR PAPER

Figure 1 Schematic illustration for gradient-hydrogel fabrication (finished)

Figure 2 Gradient improvement by replacing PBS with low-concentrated PEG solution (finished)

Figure 3 Optimization of backward flow time on gradient formation (finished)

Figure 4 PEG solution concentration on gradient formation (half done)

Figure 5 Collagne-gradient hydrogels for cell attachment (ongoing)

Figure 6 Gradient-compliant hydrogel to modulate cell behavior (ongoing)

F) FUTURE DIRECTIONS

To produce gradient tissue engineering scaffold with gradient structure

To investigate cell coculture