Fabrication of gradient hydrogels to guide cell behavior
Begin Paper Summary Template
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DATE CREATED: Jan 7, 2009
ACTION PLAN
PROBLEMS IDENTIFIED (PI) / OUTSIDE SKILL REQUIRED (OSR) / RESOLVED (R)
PAPER TITLE
A) Background.
B) Specific Aims:
C) General Experimental Approach (Design etc):
D) Design Pitfalls and alternatives
E) Potential Figures:
F) Future Directions:
A) BACKGROUND:
Fabrication of stable long-distance gradient for biological study.
B) SPECIFIC AIMS
AIM 1 - modulate cell morphology and migration
AIM 2 -
C) GENERAL EXPERIMENTAL APPROACH
Microfluidics, Cell culture
D) DESIGN PITFALLS AND ALTERNATIVES
E) POTENTIAL FIGURES FOR PAPER
Figure 1 Schematic illustration for gradient-hydrogel fabrication (finished)
Figure 2 Gradient improvement by replacing PBS with low-concentrated PEG solution (finished)
Figure 3 Optimization of backward flow time on gradient formation (finished)
Figure 4 PEG solution concentration on gradient formation (half done)
Figure 5 Collagne-gradient hydrogels for cell attachment (ongoing)
Figure 6 Gradient-compliant hydrogel to modulate cell behavior (ongoing)
F) FUTURE DIRECTIONS
To produce gradient tissue engineering scaffold with gradient structure
To investigate cell coculture