6-15-09

June 15, 2009

Won Gu:

· Journal submission to Tissue Engineering (not using personal account for submission)

· Spend time to prepare for the two papers

· Problem with the microscopes with misaligned UV source and microscopy, the life-time of UV light

Future plan:

· Mae new PDMS device

· Solve the clogging problem

· Mae a schedule for the microscopy and as help from the company

·

Dr. Sim

1. Past two weeks

· - Counting the total cell number in various-sized EB.

· - Macro-sized stimulation experiment using T-25 flask

· - Micro- sized stimulation exp. in stem cell chip

2. Next two weeks

· - RNA isolation of Macro-sized stimulation sample

· - Making hydrogel structure in the micro chamber of cell chip

· - Micro-sized stimulation experiment

Masoud& Bonggeun

· Histogram of the cell seeding in the groove with different geometries

· Plot the shear stress of the device

Suggestion and the Future plan:

· Size of pole, expect the larger the pole will cause better effects

· Density of the pole along the channel

· Considering whether the neighboring poles effect each other or not

· The shape of the pole effects cell patterning

· Figure out the title of the paper

· Show how to control the patterning profile and cell patterning profile

Jianang He and yuqi

· Show the progress Generate composite materials with tubable chemical and mechanical properties

· Calibrate the pump injection and withdrawing mode

· Gradient generation of two type of materials in the channel

· Great chatosin-gelatin composite material

Suggestion and future plan:

· Title: Controllable and tunable

· Arrange the profile figure not to give people impression that it shows the dynamics of the profile

· Clarify the application of the composite materials

· F

Seda

· Seeding cell inside the microwells

· Cardiomyocyte isolation

· ELISA system to detect of the troponin release

Suggestion and future plan:

· Confirm of the troponin release from the cells by ELISA and quantify the results

· Use microwells for cell seeding

Matt and Du

Working on the paper of the flow ossilation on the gradient generation.