6-15-09
June 15, 2009
Won Gu:
· Journal submission to Tissue Engineering (not using personal account for submission)
· Spend time to prepare for the two papers
· Problem with the microscopes with misaligned UV source and microscopy, the life-time of UV light
Future plan:
· Mae new PDMS device
· Solve the clogging problem
· Mae a schedule for the microscopy and as help from the company
·
Dr. Sim
1. Past two weeks
· - Counting the total cell number in various-sized EB.
· - Macro-sized stimulation experiment using T-25 flask
· - Micro- sized stimulation exp. in stem cell chip
2. Next two weeks
· - RNA isolation of Macro-sized stimulation sample
· - Making hydrogel structure in the micro chamber of cell chip
· - Micro-sized stimulation experiment
Masoud& Bonggeun
· Histogram of the cell seeding in the groove with different geometries
· Plot the shear stress of the device
Suggestion and the Future plan:
· Size of pole, expect the larger the pole will cause better effects
· Density of the pole along the channel
· Considering whether the neighboring poles effect each other or not
· The shape of the pole effects cell patterning
· Figure out the title of the paper
· Show how to control the patterning profile and cell patterning profile
Jianang He and yuqi
· Show the progress Generate composite materials with tubable chemical and mechanical properties
· Calibrate the pump injection and withdrawing mode
· Gradient generation of two type of materials in the channel
· Great chatosin-gelatin composite material
Suggestion and future plan:
· Title: Controllable and tunable
· Arrange the profile figure not to give people impression that it shows the dynamics of the profile
· Clarify the application of the composite materials
· F
Seda
· Seeding cell inside the microwells
· Cardiomyocyte isolation
· ELISA system to detect of the troponin release
Suggestion and future plan:
· Confirm of the troponin release from the cells by ELISA and quantify the results
· Use microwells for cell seeding
Matt and Du
Working on the paper of the flow ossilation on the gradient generation.